locus

BSU23070

BSU_23070

outlinks

bsu

BSU23070

BSU_23070

The protein

Catalyzed reaction/ biological activity

3-phospho-D-glycerate + NAD⁺ = 3-phosphonooxypyruvate + NADH (according to Swiss-Prot)

3-phospho-D-glycerate + NAD⁺ --> 3-phosphooxypyruvate + H⁺ + NADH (according to UniProt)

(R)-2-hydroxyglutarate + NAD⁺ --> 2-oxoglutarate + H⁺ + NADH (according to UniProt)

The protein

Protein family

D-isomer specific 2-hydroxyacid dehydrogenase family (according to Swiss-Prot)

D-isomer specific 2-hydroxyacid dehydrogenase family (with [[protein|YvcT]] and [[protein|YoaD]], according to UniProt)

The protein

[SW|Domains]

[SW|ACT domain] (aa 452 ... 524) (according to the Interpro database)

[SW|ACT domain] (aa 452-524) (according to UniProt)

Biological materials

Mutant

1A614 ( ''serA''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A614&Search=1A614 BGSC] and in [SW|Jrg Stlke]'s lab

BKE23070 ([[gene|serA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTAGATTTCCTCCTA, downstream forward: _UP4_TAATTAAAAAAACTCAAGCT

BKK23070 ([[gene|serA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTAGATTTCCTCCTA, downstream forward: _UP4_TAATTAAAAAAACTCAAGCT

GP2392 ∆''[[gene|serA]]''::''zeo'', available in [SW|Jörg Stülke]'s lab [pubmed|32743959]

1A614 (''serA''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A614&Search=1A614 BGSC] and in [SW|Jörg Stülke]'s lab

BKE23070 ([[gene|serA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTAGATTTCCTCCTA, downstream forward: _UP4_TAATTAAAAAAACTCAAGCT

BKK23070 ([[gene|serA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTAGATTTCCTCCTA, downstream forward: _UP4_TAATTAAAAAAACTCAAGCT

Biological materials

lacZ fusion

pGP187 (in [[protein|pAC7]]), available in [SW|Jrg Stlke]'s lab

pGP187 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

pGP2287 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

References

22938038, 17611193, 7934830, 18763711, 15823035, 21749987, 23033921, 15378759

22938038, 17611193, 7934830, 18763711, 15823035, 21749987, 15378759, 31138626, 32743959

Gene

Phenotypes of a mutant

auxotrophic for serine [pubmed|32743959]